Prediction of the in-vitro fertilization (IVF) potential of human spermatozoa using sperm function tests: the effect of the delay between testing and IVF.
نویسندگان
چکیده
To examine the diagnostic significance of several criteria of semen quality and to determine whether their prognostic value is eroded by the time interval between assessment and the attempt at in-vitro fertilization (IVF) with embryo transfer, 73 couples undergoing IVF and embryo transfer therapy were studied. The ability of human spermatozoa to achieve fertilization in vitro was examined in relation to the conventional semen profile, sperm morphology, the computer-aided assessment of sperm movement, ionophore-induced acrosome reaction, acridine orange staining, and chemiluminescent signals induced by phorbol ester and N-formyl-methionyl-leucyl-phenylalanine (FMLP). Spermatozoa were examined both in semen and after preparation on Percoll, some weeks prior to IVF. Fertilization rates were noted to be significantly correlated with elements of sperm movement characteristics, sperm morphology, and reactive oxygen species generation. Prediction of fertilization rates in a stepwise multiple regression analysis was obtained using four variables: sperm morphology, FMLP-induced chemi-luminescence and sperm movement characteristics (beat cross frequency and straightness) (r approximately 0.5). When multiple logistic regression analysis was used to predict which samples would achieve fertilization rates above and below a 50% threshold, three variables of predictive value including linearity, average path velocity and FMLP-induced chemiluminescence were selected. Combination of these variables classified the samples achieving good or poor fertilization with an overall accuracy of 83.6%. The time interval between semen assessment and IVF had little effect on the predictive value of these tests. In conclusion, the fertilizing ability of human spermatozoa is related to sperm morphology, attributes of sperm movement and reactive oxygen species production. The time delay between testing and IVF did not appear to affect predictive accuracy.
منابع مشابه
Evaluation of frozen thawed cauda epididymal sperms and in vitro fertilizing potential of bovine sperm collected from the cauda epididymal
In the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. The cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. Sperm fertility potential was tested...
متن کاملP-109: Effect of Chaste Tree Essential Oil on Sperm In Vitro Fertilization (IVF) Index in Mice
Background: Chaste tree is a phytoestrogenic herb and has a long tradition as a herbal remedy and was used in ancient times as an aphrodisiac. This study was conducted to evaluate in vitro fertilization ability and cleavage rate of sperm in chaste tree essential oil treated male mice. Materials and Methods: In this study, 10 adult male mice were divided in to two groups: group 1 as control and ...
متن کاملP-67: Reactive Oxygen Species, MDA and NO Concentration of Testicular Extract and Their Effect on Sperm Parameters and IVF Outcome in Hyperprolactinemic Adult Male Mice
Background: ROS are free radicals that have a significant role in many of the sperm physiological processes. In the presence of polyunsaturated fatty acids such as sperm plasma membrane,ROS triggers a chain of chemical reactions called lipid peroxidation.The aim of this study was to determine and compare the concentration of MDA and NO in testicular extract of hyperprolactinemic adult male mice...
متن کاملO-30: The Correlation between Myometrial Thickness and In Vitro Fertilization/ Intracyto- Plasmic Sperm Injection (IVF/ ICSI) Outcomes
Background O-30: The Correlation between Myometrial Thickness and In Vitro Fertilization/ Intracyto- Plasmic Sperm Injection (IVF/ ICSI) Outcomes MaterialsAndMethods In this prospective cohort study, 453 infertile women undergoing IVF-ET/ICSI cycles without any obvious pathology of uterine were enrolled. Trans-vaginal ultrasounds were conducted on days 2-4 of the cycle (menstrual phase) precedi...
متن کاملEffects of Sperm Acrosomal Integrity and Protamine Deficiency on In Vitro Fertilization and Pregnancy Rate
Background The objective of this study was to evaluate the relationship between protamine deficiency, and acrosomal integrity with fertilization and pregnancy rate in patients undergone in vitro fertilization (IVF). MaterialsAndMethods Semen samples from 70 infertile couples undergoing IVF at Isfahan Fertility and Infertility center were assessed in this study. Semen analysis was carried out ac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Human reproduction
دوره 11 5 شماره
صفحات -
تاریخ انتشار 1996